Effect of light intensity on in vitro multiple shoot induction and regeneration of cotton (Gossypium hirsutum L. cv Khandawa-2).

Effect of Kinetin on Multiple Shoot Induction in Cotton (Gossypium hirsutum L.) cv. NIAB-999

In vitro Plant Regeneration from the Callus of Shoot Tips in Cotton (Gossypium hirsutum L. cv. SVPR 2)

A high efficiency shoot organogenesis from callus cultures and plant establishment protocol has been developed for cotton (Gossypium hirsutum L. cv SVPR-2) through shoot tip. After 6 weeks of culture, green-compact-nodular organogenic callus induction was observed on the medium fortified with (MS) basal salts, B5 vitamins, 2.0 mgl-1 of benzylaminopurine (BAP), 1.0 mgl-1 kinetin (KN), 30 gl-1 gl...

باززایی 6 ژنوتیپ پنبه (Gossypium hirsutum L.) از طریق جنین زایی سوماتیکی

Optimization of regeneration methods in cotton is necessary to improve and successful production of transgenic cotton cultivars by tissue culture techniques. Present study was conducted to determine the callus induction and plant regeneration of cotton genotypes through In vitro culture and this carried out using hypocotyl and cotyledon explants from 7-14 day-old. For callus induction the expla...

Comparison of oil and fatty acid composition of seed, shoot and in-vitro regenerated organs of cotton (Gossypium hirsutum L. var. Sahel)

Cotton (Gossypium hirsutum L.) is a source of edible oil and fatty acids (FAs). In this study, embryos’ explants were cultured in MS medium with different concentration of  plant growth regulators (phytohormones) include BA, IAA, IBA, 2, 4- D and NAA, to finding the relationship of hormone and organogenesis with oil and FAs content. Oil was extracted with chloroform and methanol. FAs o...

High-frequency regeneration via multiple shoot induction of an elite recalcitrant cotton (Gossypium hirsutum L. cv Narashima) by using embryo apex

Cotton (Gossypium hirsutum L.) is one of the most commercially important fiber crops in the world. Compared with other crops, cotton represents a recalcitrant species for regeneration protocols. The development of efficient and rapid regeneration protocol for elite Indian cotton variety could help improve the quality characteristics and biotic or abiotic stress tolerance. Here we report a novel...